The analysis of the expression of markers of apoptosis and proliferation in 80 patients with ulcerative colitis has been carried out. The reference group consisted of 15 practically healthy people. In biopsy materials of the colonic mucosa the expression of immunohistochemical markers Ki-67 and p53 was determined. The reduction of the proliferative activity of epithelial cells of the colon and increase in the activation of apoptosis with the growing severity of ulcerative colitis, the increase of the colon lesion range and the disease endoscopic activity has been revealed.
Anna A. Markova and Elena I. Kashkina Expression of markers of apoptosis and proliferation in patients with nonspecific ulcerative colitis // Russian Open Medical Journal, Vol. 1, Issue 2, 2012, pp. 206-0

Aim: to study the patterns of changes in the number of DNA double-strand breaks (DSB) in mammalian cells continuously exposed to low dose-rate y- radiation. Material and methods. Chinese hamster lung fibroblasts (V79) were used in this study. The y- irradiation of cells at a dose rate of 0.1 mGy/min was performed using the «Gamma-Panorama» unit (Cs-137). The fluorescence immunoassay of the phosphorylated H2AX-histone (y-H2AX) foci was used to investigate the DNA DSBs formation. Frequency of apoptotic cells was evaluated using «DNA halo» assay. 5 (6) — chloromethyl-2,7-dichlorodihydrofluorescein diacetate was used to estimate the reactive oxygen species (ROS) production. Results, it was showed that continuous low dose-rate irradiation of Chinese hamster V79 cells induces an increase of the y-H2AX foci number and ROS production rate at the early stages of exposure time (6-24 h, doses 3.6-14.4 cGy), while increasing exposition time and, therefore, the radiation dose (48-72 h, 28.8-43.2 cGy) caused a decrease in these endpoints to almost the control level. There was observed no significant changes in the frequency of apoptotic cells. Conclusion. It is assumed that the processes causing the DSB amount changes in mammalian cells continuously exposed to low dose-rate y-radiation are associated with the development of oxidative stress and subsequent activation of cellular antioxidant defense systems.
Ozerov I.V., Bushmanov A.Yu., Anchishkina N.A., Guryev D.V., Pustovalova M.V., Smetanina N.M., Arkhangelskaya E.Yu., Vorobyova N.Y., Osipov A.N. Induction and reparation of double-strand DNA breaks in V79 cells continuously exposed to low dose-rate Y-radiation // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 4, 2013, pp. 787-791

The aim of the research was the study of the cell proliferation indices of the gum epithelial cells in patients with parodentium inflammatory diseases accompanied by gastroesophageal reflux disease (GERD). Material and methods. 160 patients were examined, 60 patients with parodentium inflammatory diseases accompanied by NEGERD, 40 patients with parodentium inflammatory diseases accompanied by GERD. The comparison groups consisted of 40 patients with diseases of parodentium without esophagus pathology and 20 healthy persons. Results. On the basis of the conducted clinical-endoscopic, immune-histological and morphological examinations it was established that gingivitis and periodontitis are successive stages of disturbance of the cellular homeostasis of gum epithelial cells. Conclusion. The enhancement of the processes of proliferation and apoptosis promotes the progression of the structural alterations in the tissues of parodentium and oesophagus and may serve as additional diagnostic and prognostic criteria of the diseases of parodentium accompanied by GERD.
Osipova J.L., Bulkina N.V., Osadchuk М.А., Kvetnoy I.M. The role of epithelial cells proliferation and apoptosis disturbances in pathogenesis of inflammatory diseases of parodentium accompanied by gastroesophageal reflux disease // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 3, 2013, pp. 449-453

The Research Objective: Correlation of apoptosis and senilism in patients with metabolic syndrome has been studied. Materials. Apoptosis inductor protein p53 concentration, insulin level in blood serum, biological age, ageing rate coefficient of 270 patients with metabolic syndrome of the age from 30 till 60 have been determined. Among them there have been 162 men and 108 women. Ageing rate coefficient and biological age have been calculated by A. G. Gorel-kin's and Pinchasov's formulae, protein p53 concentration and insulin in blood serum have been determined by enzyme multiplied immunoassay method. Results. Protein p53 concentration of the patients with metabolic syndrome has been increased and worked out 1,59 (1,361,91) U/ml, biological age, ageing rate coefficient have been increased — biological age to 6 years, ageing rate coefficient has been 1,32. Conclusion. These patients have been referred to the group with high risk of early pathology development because the processes of senilism are proleptic. The direct connection between protein p 53 and ageing rate coefficient (r+0,63, p< 0,05) and between apoptosis marker protein p53 and biological age (r+0,6, p< 0,05) have been determined.
Chernysheva E.N., Panova T.N. Apoptosis and premature aging in patients with metabolic syndrome // Saratov Journal of Medical Scientific Research, Vol. 8, Issue 2, 2012, pp. 251-255

The aim of the research is to study and to analyze results of investigations of cytokine blood profile in patients with B-cellular chronic lymphocytic leukaemia. Materials and methods: Complex investigation of 60 patients with B-cellular chronic lymphocytic leukaemia has been carried out. Patients have been randomized into 4 groups of observation according to the severety of disease (classification of Rai K. R., 1975). Accepted methods of somatic status and he-matologic indices as well as method of flow cytometry for establishment of B-lymphocytes immune phenotype have been used. Cytokine levels were detected by enzyme immunodetection. Results: Regular peculiarity of cytokine status change in different forms of chronic lymphocytic leukaemia development has been an increase of interleukine-4, interleukine-6, interleukine-7, interleukine-10 and tumor necrosis factor-alpha levels in serum that is one of the main pathogenetic factor of intercellular interaction abnormality in lymphoid tissue in the chronic lymphocytic leukaemia development. Conclusion: Increased indications of cytokines content in blood may be used as diagnostic criteria of pathology severity and in evaluation of effectiveness of complex therapy of the disease, as well as a classification sign of severity of chronic lymphocytic leukaemia
Zhevak T.N., Chesnokova N.P., Shelekhova T.V. Regularities of cytokine status changes in chronic lymphocytic leukaemia and their role in pathogenesis of progressive forms of disease // Saratov Journal of Medical Scientific Research, Vol. 8, Issue 2, 2012, pp. 203-209

The research work presents an analysis of literature review and results of investigations on the problems of etiology, pathogenesis, classification and peculiarities of clinical manifestations of chronic lymphocytic leukaemia. Special attention is paid to both contemporary conceptions of carcinogenesis, reflecting the role of proto-oncogenes activation, an-tioncogenes and apoptosis genes suppression in mechanisms of neoplasia development and «specific mechanisms» of oncogenic transformation induction of B-lymphocytes and the subsequent development of the stages of promotion, progression and marked clinical manifestations of formation. Possibility of further improvement of use of immune phe-notype character and oncomarkers in diagnostics of chronic lymphocytic leukaemia clinical variants and in evaluation of effectiveness of its therapy has been indicated in the article
Zhevak T.N., Chesnokova N.P., Shelekhova T.V. Chronic lymphocytic leukaemia: contemporary conceptions of etiology, pathogenesis and peculiarities of clinical manifestations (review) // Saratov Journal of Medical Scientific Research, Vol. 7, Issue 2, 2011, pp. 376-385

Глутатіон—S-трансфераза Р1-1 (GSTP1-1) є багатофункціональним ензимом, який захищає клітину від дії генотоксичних чинників та апоптозу. У цьому огляді узагальнено сучасні дані щодо будови молекули глутатіон—S-трансферази Р1-1 та його функцій, показано зв’язки між його структурними особливостями та каталітичними властивостями. Порівнюються структурно-функціональні особливості ізоформи Р1 з іншими глутатіон—S-трансферазами. Головний акцент в огляді зроблено на нещодавно відкритих властивостях ензиму модулювати роботу сигнальних шляхів та забезпечувати внутрішньоклітинне зберігання і метаболізм оксиду азоту. Glutathione S-transferase P1-1 (GSTP1-1) is a multifunctional enzyme which protects the cell from the influence of genotoxic factors and apoptosis. Contemporary knowledge about the GSTP1 molecule structure and the enzyme functions are summarized in this review. Also P1 isoform is compared to other glutathione S-transferases and structure-function relationships are emphasized. Novel functions of GSTP1 in cell signaling modulation, NO storage and metabolism are highlighted in this paper.

Purpose — Studying of kliniko-morphological features of a pathology of a gullet, stomach, duodenal gut and role of indicators apoptosis and proliferation in pathology development esofagogastroduodenal zones at alcoholic liver disease. 42 patients with an alcoholic hepatitis and 44 patients a cirrhosis are surveyed. By all it is spent endoscopic, histo-logic and immuno-histochemical inspection. By means of sets of antibodies studied proliferation activity epitheliocytes of stomach At patients at transformation of a chronic alcoholic hepatitis in a cirrhosis accrue atrophy changes of a mucous membrane of a stomach, against against which chronic erosion or peptic ulcers without inflammations are marked. Processes proliferation and apoptosis are activated, that to the full defines morphological changes gastroduodenal zones Strengthening of processes proliferation and apoptosis promotes progressing of structural changes esophagogas-tricduodenal zones that can serve additional diagnostic and prognostic as criterion.
Sushchenko M.A., Kozlova I.V. Klinico-diagnostic value of indicators apoptosis and proliferation esofagogastroduode-nal zones at alcoholic liver disease // Saratov Journal of Medical Scientific Research, Vol. 5, Issue 3, 2009, pp. 347-352

This research is dedicated to the problem of choosing treatment tactics in catarrhal form of acute appendicitis. The results of investigation and treatment of 1468 patients with acute appendicitis of our region were analyzed. Large specific gravity (26%) of patients who had been operated with regard to acute appendicitis was exposed. With the purpose of improvement of surgical treatment results of patients with acute appendicitis we have worked out and instilled a diagnostic system, which is a totality of invasive and uninvasive methods of investigation that allows to divide clinical material into two categories: one of them is with existence of inflamed process and the other is without it.
S.S. Slesarenko, A.Yu. Lisunov FEATURES OF SURGICAL TACTICS AND TREATMENT OF ACUTE APPENDICITIS AT PRESENT STAGE // Saratov Journal of Medical Scientific Research, Vol. 4, Issue 3, 2008, pp. 111-118