The aim of the study is a comparative evaluation of the diagnostic value of determining of concentration of cyto-kines in the serum and urine of patients with acute pyelonephritis. Materials and Methods: The study of cytokine profile in blood serum and urine has been performed in 21 patients with acute pyelonephritis aged from 23 to 63 years. The concentration of interleukin (IL-1 p, IL-6, IL-8, IL-10) receptor antagonist, IL-1 (IL-1RA), tumor necrosis factor — alpha (TNF-a), C-reactive protein (CRP), in serum and urine of patients under the survey have been determined by the enzyme-linked immunosorbent assay (ELISA) using the appropriate reagent sets «Vector-Best» (Novosibirsk). The study of all these biomarkers in patients with acute pyelonephritis has been performed three times: at admission before antibiotic therapy (1 point); in a 5-7 days-period from the beginning of treatment (2 points); 1.5 months after completion of treatment (3 points,). Results: Before the treatment there has been an increase in levels of IL-6, IL-8, IL-1RA, TNF-a, CRP in serum. Simultaneously, the concentration of these substances in the urine has been higher. In 2 and 3-point changes the level of cytokines has been different; however, the content of them in the urine has been determined to be higher than in the serum. Conclusion: Analysis of changes in levels of cytokines and C-reactive protein in serum and urine showed that these biomarkers in acute pyelonephritis may be used as indicators of inflammatory activity. The data received suggest that the study of cytokine levels in the urine may be used to assess the severity of inflammatory changes in the renal parenchyma and in monitoring of therapy effectiveness.
Popkov V.M., Dolgov А.В., Zakharova N.B., Ponukalin A.N., Varaksin N.A. Urinary biomarkers in acute pyelonephritis // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 1, 2013, pp. 110-115

Chronic hepatites for today remain the important problem with children among children»s infections not only in our country, but also all over the world. In clause the immune factors influencing on патогенез of a chronic virus hepatites В at children are considered. The purpose of our job was studying interrelation cytolysis and a structure of cytokines at children sick of a chronic virus hepatites В Century. Material, methods. We have carried out research a structure of cytokines at 87 children with CVH В in the age of from 7 months till 18 years. Clinical supervision and researches with application general-clinical, biochemical, tool, immunological the methods have been spent, allowing to receive the fullest and objective information on the observable children sick CVH. Results. If to compare parameters cytokines in replicative and integration phases at a chronic virus hepatites В at children it is necessary to note, that in replica-tive a phase the basic parameters are aggravated. The conclusion. It speaks that in replicative phase CVH disease more hard proceeds. The deviation of level FNO-a, IL-1, IL-10 cytokines in whey of blood of patients with CVH В from normal parameters is the indicator of gravity of inflammatory process
Keltsev V.A., Churbakova О.V. Interrelation of cytokine profile and cytolysis in children with chronic virus hepatitis В // Saratov Journal of Medical Scientific Research, Vol. 7, Issue 2, 2011, pp. 461-465

The research work presents an analysis of literature review and results of investigations on the problems of etiology, pathogenesis, classification and peculiarities of clinical manifestations of chronic lymphocytic leukaemia. Special attention is paid to both contemporary conceptions of carcinogenesis, reflecting the role of proto-oncogenes activation, an-tioncogenes and apoptosis genes suppression in mechanisms of neoplasia development and «specific mechanisms» of oncogenic transformation induction of B-lymphocytes and the subsequent development of the stages of promotion, progression and marked clinical manifestations of formation. Possibility of further improvement of use of immune phe-notype character and oncomarkers in diagnostics of chronic lymphocytic leukaemia clinical variants and in evaluation of effectiveness of its therapy has been indicated in the article
Zhevak T.N., Chesnokova N.P., Shelekhova T.V. Chronic lymphocytic leukaemia: contemporary conceptions of etiology, pathogenesis and peculiarities of clinical manifestations (review) // Saratov Journal of Medical Scientific Research, Vol. 7, Issue 2, 2011, pp. 376-385