The present study was designed to investigate changes in angiotensin-converting enzyme (ACE) blood activity and angiotensin II type 1 receptor gene polymorphism as a possible disease predictor in hemorrhagic fever with renal syndrome (HFRS). Four hundred and nine patients (346 males and 63 females) with HFRS serologic confirmation were enrolled in the study. Their age ranged from 15 to 65 years. ACE blood activity was assessed kinetically using the Bühlmann (Switzerland) kit. Peripheral blood genomic DNA was isolated by a phenol-chloroform extraction. The genotyping of DNA loci was done using a polymerase chain reaction of DNA synthesis. Statistically, ACE blood activity was significantly higher throughout the entire HFRS course with diverse severity apart from the feverish phase of moderate-to-severe uncomplicated disease forms. *A1166 and *C1166 alleles, *A1166/*A1166 and *C1166/*C1166 genotypes of angiotensin II type 1 receptor gene were not associated with HFRS severity. The results of this study indicate that high ACE activity has not adaptive characteristics due to abnormalities in angiotensin II reception. It is an adequate metabolic response of the body to endotheliotropic virus activity.
Asiya A. Baygildina, Denis V. Islamgulov, Felix Kh. Kamilov, Tamara A. Khabelova, Ildar R. Minniakhmetov Molecular-genetic risk assessement of determining angiotensin-converting enzyme hyperactivity in hemorrhagic fever with renal syndrome // Russian Open Medical Journal, Vol. 1, Issue 2, 2012, pp. 201-0

The research goal is to examine the interrelation of cytokines level (INF-y, TNF-a, IL-2, IL-4, IL-10) and concentration of microelements in blood of patients with hemorrhagic fever accompanied by renal syndrome. 292 patients aged 18-59 years have been examined.Enzyme immunodetection has been carried out by using reagents from «Vector-Best» (Novosibirsk). The content of microelements in blood plasma has been estimated by mass spectrometry with inductively coupled plasma (ICP-MS; Elan-9000, PerkinElmer, USA) and atomic emission spectrometry with inductively coupled plasma (ICP-OES; Optima-2000 DV, PerkinElmer, USA). A direct correlation between zinc, selenium, the level of IL-2 and lead, aluminum, the level of TNF-a has been found out. The negative correlation between aluminum concentration and the level of IF-y has been determined
Khasanova G.M. Level of circulating cytokines and microelements in patients with hemorrhagic fever accompanied by renal syndrome // Saratov Journal of Medical Scientific Research, Vol. 7, Issue 4, 2011, pp. 863-865

The research goal is to determine the changes in concentration of both sVCAM-1 and VE-cadherin in blood serum of patients suffered from hemorrhagic fever with renal syndrome (HFRS). 87 patients aged 15-65 were examined. Concentrations of both sVCAM-1 and VE- cadherin in blood serum by means of "Bender MedSystems" (Austria) ELISA test were determined. It was shown that in both medium severe and severe forms of HFRS statistically the significant rise of sVCAM-1 concentration in blood with high indices in oliguric period took place. Complicated form was characterized by high indices of sVCAM-1 level in fever period, extremely decreasing in concentration in oliguric period and tendency to normalizing in clinical convalescence period. VE-cadherin level in blood was predominantly lower than control in all the observed groups with the exception of fever period in group with medium severe disease form. Negative correlation of normal intensity between adhesion molecules levels in blood was revealed. In conclusion it is necessary to point out that high VCAM-1 expression by endotheliocytes evidences the development of an adhesion form of endothelial dysfunction, low VE-cadherin production in a base for development of angiogenic form of endothelial dysfunction and changes in expression of these adhesion molecules that have adaptive metabolic response to macroorganism of HFRS pathogenic action
А.А. Baygildina Role Of Adhesion Molecules Vcam-1 And Ve-Cadherin In Endothelium Dysfunction Development At Hemorrhagic Fever With Renal Syndrome // Saratov Journal of Medical Scientific Research, Vol. 5, Issue 4, 2009, pp. 478-481