Objective: assessment of dynamics of reparative processes with the help of cytological examination of the preparations obtained from a conditionally aseptic wound under the action of a complex preparation based on biopolymer chitosan and metal nanoparticles. Material and Methods. A conditionally aseptic wound was simulated in 40 white outbred rats. In comparison and experimental groups cytologic assessment of dynamics of reparative processes was carried out. A number of neutrophilic leukocytes and fibroblastic row perl 00 cells in the preparations is recorded on the 3rd, 5th, 7th, 10th, 14th day from the moment of formation of the wound with the use of the immersion objective. Results. It is established, that in the comparison group the cytologic pattern of impression smear was characterized by decrease in neutrophilic leukocytes and increase in fibroblastic row cells by the 14th day from the moment of formation of the wound. In the experimental group (the use of a complex preparation) more intensive reduction of neutrophilic leukocytes and a significant increase in fibroblastic row cells by the 10th day and a complete healing by the 14th day from the moment of formation of the wound has been determined. Conclusion. When treated by a complex preparation based of biopolymer chitosan and metal nanoparticles earlier occurrence of fibroblastic row cells in preparations and a significant decrease in quantity of neutrophilic leukocytes have been observed.
Konyuchenko Е.А.. Zhandarova L.F., Babushkina I.V., Gladkova E.V., Mamonova I.A., Belova S.V., Karyakina E.V. Cytologic assessment of dynamics of reparative processes in the experimental conditionally aseptic wound under the action of complex preparation based on chitosan and metal nanoparticles // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 4, 2013, pp. 629-632

Purpose: to study the properties of nanostructured coatings in orthodontic implants. Material and methods. Low and average molecular mass chitosan, 3-amynopropil-3-methoxysylan and suspensions of 5 nm and 10 nm gold nano-spheres stabilized with natrium citrate were used for nanocomposed surfaces. 2mg/ml polyethylenamin water solution was used for making the underlayer before putting polyion coverage using «POLYION-1M». The polyion covering dynamics was studied by polyquartz weighing method. Morphology of created layers was studied with atomic microscopy, elements were studied by secondary ion mass-spectrometry. Results. The best transmission among structures of chito-san/metals was showed by low molecular mass of chitosan and 8-1 Onm nanoparticles of metals. Analysis of roughness of surface shows that nanoparticles of gold make the most solid surface on 3-amynopropil-3-methoxysylan underlayer. Conclusion. The development of biocomparative materials in maxillofacial surgery is considered to be effective method of decreasing the risk of post-operative inflammatory complications by local antibacterial effect.
Suetenkov D.Ye., Gorin D.A., Portnov S.A., Karagaychev A.L., Terentyuk G.S., Zakharevich A.M., Khlebtsov B.N., Firsova I.V. Surface modification of orthodontic implants by nanocomposite coatings based on chitosan and metals // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 3, 2013, pp. 472-477

Polyoxometalates (POMs) show great molecular diversity and have significant applications in material science as well as in medicine. In this study, nano-complexation of a novel europium containing polyanion [Cs⊂Eu6As6W63O218(H2O)14(OH)4]25− (EuWAs) with biocompatible chitosan was achieved through ionotropic gelation technique without the aid of any cross-linker. Thus obtained chitosan/EuWAs nano-complex was characterized using DLS and Zeta analysis, FT-IR, SEM, AFM, TG/DTA, EDAX and fluorescence spectroscopy. The cross-linking efficiency of EuWAs with chitosan was calculated to be 81% and the release profile recorded at physiological pH was slow and sustained. Cytotoxicity assays performed on a host of cancer cell lines, viz., KB, MCF-7, PC-3 and A549 proved the anticancer activity of the nanocomplex and flow cytometry studies revealed that reactive oxygen species generation can be the plausible mechanism for the apoptosis induced by this material. Our study has thus indicated the feasibility of using chitosan/EuWAs nano-complex for anticancer applications.

Medicinal form with all its components: auxiliary substances, way of introduction of medicinal substances, favourable properties, appearance and convenience of application influences its therapeutic efficiency. Biological availability of heterogeneous medicinal forms for peroral applications in the form of suspension and emulsion were compared in the research. There were included natural auxiliary substances. Comparative investigations were held on models of experimental gastric ulcer on Okabe and single introduction of hormones. The presented research stated that medical products as components of heterogeneous, correct medicines in the form of suspensions and emulsions positively influence the therapeutic efficiency. Besides, the substances stabilizing heterogeneous systems intensify and supplement pharmacological action of medical product
Pantyukhin A.V. Heterogeneous peroral medicinal forms in treatment of digestive disorders // Saratov Journal of Medical Scientific Research, Vol. 6, Issue 4, 2010, pp. 762-766

The copper(II) complex formation equilibria of N-(2-carboxyethyl)chitosans with three different degrees of substitution (DS ) 0.42, 0.92, and 1.61) were studied in aqueous solution by pH-potentiometric and UVspectrophotometric techniques. It was demonstrated that the complexation model of CE-chitosans depends on DS: the [Cu(Glc-NR2)2] complexes are predominant for two lower substituted samples (“bridge model”, log â12 ) 10.06 and 11.6, respectively), whereas the increase of DS leads to formation mainly of the [Cu(Glc-NR2)] complexes (“pendant model”, log â11 ) 6.41). As a model for copper complexation with a disubstituted residue of CE-chitosan, the complex of N-methyliminodipropionate [CuMidp(H2O)]â(H2O) was synthesized and structurally characterized by XRD. The unit cell consists of two crystallographically nonequivalent Cu atoms having slightly distorted square pyramidal coordination; Midp constitutes the basal plane of the pyramid and acts as a tetradentate NO3 chelate-bridging ligand by the formation of two sixmembered chelate rings (average Cu-O 1.99 Å, Cu-N 2.04 Å) and a bridge via carbonyl O atom (average Cu-O 1.99 Å), an apical position is occupied by a water molecule (average Cu-Ow 2.30 Å).
Biomacromolecules. 2005 6(1) 189-195

N-carboxyethylation of chitosan by b-halopropionic acids in the presence of various proton and halogen ion acceptors was investigated. It has been observed that carboxyethylation of chitosan in aqueous medium is accompanied by the by-processes of hydrolysis and dehydrohalogenation of the b-halopropionic acids yielding b-hydroxypropionic acid, bis(2-carboxyethyl) ether, and acrylic acid. Degree of carboxyethyl substitution (DS) of chitosan and the relative rates of the by-processes varied significantly depending on the conditions used and nature of the proton or halogen ion acceptor. At carboxyethylation of chitosan with the alkaline b-bromopropionates, the DS increased in the order Csþ 5 Rbþ 5 Kþ
Naþ 5 Liþ. For alkaline earth salts BrCH2CH2COOM0.5 (M 5 Be2þ, Mg2 þ, Ca2 þ, Sr2 þ, Ba2 þ), the highest DS was obtained with strontium and barium salts, which could be subsequently removed from the reaction mixture by precipitation as sulfates. Among the organic bases applied (tetrabutylammonium hydroxide, triethylamine, trimethylamine, pyridine, 4-N,N-dimethylaminopyridine, 2,6-lutidine, and 1,5-diazabicyclo[4.3.0] non-5-ene), the highest DS was obtained using a moderately strong base triethylamine. For the halogen acceptors (Pb2þ, Agþ, Tlþ), the stoichiometrically highest DS was achieved in a system comprising iodopropionic acid plus Tlþ and a comparable conversion rate was obtained using also a combination of chloropropionic acid and Agþ. A novel alternative preparative approach—gel-state synthesis—was suggested that provides for the highest DS at the optimum reaction conditions.
Journal of Applied Polymer Science. 2008 108(1) 119-127.