Найдено научных статей и публикаций: 1, для научной тематики: Kamchatka crab Arthropod Sugar non-specific nucleases Mutagenesis Phylogenetic analysis
1.
Veronika E. Anisimova, Alex S. Shcheglov, Ekaterina A. Bogdanova, Denis V. Rebrikov, Alexei N. Nekrasov, Ekaterina V. Barsova, Dmitry A. Shagin, Sergey A. Lukyanov
- Gene , 2010
Kamchatka crab duplex-specific nuclease (Par_DSN) has been classified as a member of the family of DNA/
RNA non-specific beta-beta-alpha metal finger (bba-Me-finger) nucleases, the archetype of which is the
nuclease from Serratia marcescens. Although the enzyme under investigation seems to belong ...
Kamchatka crab duplex-specific nuclease (Par_DSN) has been classified as a member of the family of DNA/
RNA non-specific beta-beta-alpha metal finger (bba-Me-finger) nucleases, the archetype of which is the
nuclease from Serratia marcescens. Although the enzyme under investigation seems to belong to the family of
S. marcescens nucleases, Par_DSN exhibits a marked preference for double-stranded DNA as a substrate and
this property is unusual for other members of this family. We have searched other Arthropod species and
identified a number of novel Par_DSN homologs. A phylogenetic analysis demonstrates that the Par_DSN-like
enzymes constitute a separate branch in the evolutionary tree of bba-Me-finger nucleases. Combining
sequence analysis and site-directed mutagenesis, we found that Par_DSN and its homologs possess the
nuclease domain that is slightly longer than that of classic Serratia relatives. The active site composition of
Par_DSN is similar but not identical to that of classic Serratia nucleases. Based on these findings, we proposed
a new classification of Par_DSN-like nucleases.
Gene (2008) vol. 418, pp. 41- 48