We investigate the impact of charm mixing on the model-independent gamma measurement using Dalitz plot analysis of the three-body D decay from B+->DK+ process, and show that ignoring the mixing at all stages of the analysis is safe up to a sub-degree level of precision. We also find that in the coherent production of D0-D0* system in e+e- collisions, the effect of charm mixing is enhanced, and propose a model-independent method to measure charm mixing parameters in the time-integrated Dalitz analysis at charm factories.

Protein hydrogel-based microchips are being developed for high-throughput evaluation of the concentrations and activities of various proteins. To shorten the time of analysis, the reaction-diffusion kinetics on gel microchips should be accelerated. Here we present the results of the experimental and theoretical analysis of the reaction-diffusion kinetics enforced by mixing with peristaltic pump. The experiments were carried out on gel-based protein microchips with immobilized antibodies under the conditions utilized for on-chip immunoassay. The dependence of fluorescence signals at saturation and corresponding saturation times on the concentrations of immobilized antibodies and antigen in solution proved to be in good agreement with theoretical predictions. It is shown that the enhancement of transport with peristaltic pump results in more than five-fold acceleration of binding kinetics. Our results suggest useful criteria for the optimal conditions for assays on gel microchips to balance high sensitivity and rapid fluorescence saturation kinetics.