Связанные научные тематики:
yeast
Найдено научных статей и публикаций: 2, для научной тематики: ATP
1.
Prasenjit Mahato, Amrita Ghosh, Sanjiv K. Mishra, Anupama Shrivastav, Sandhya Mishra and Amitava Das
- Chem. Commun., 2010, 46, 9134-9136 , 2010
Selective colorimetric detection of ATP in physiological conditions by a Zn(II)-based receptor is reported. This reagent was found to be non-toxic to the living cells and could be used for studying the growth of the yeast cells....
Selective colorimetric detection of ATP in physiological conditions by a Zn(II)-based receptor is reported. This reagent was found to be non-toxic to the living cells and could be used for studying the growth of the yeast cells.
2.
Amrita Ghosh, Anupama Shrivastav, D. Amilan Jose, Sanjiv K. Mishra, Chandrakanth. C. K., Sandhya Mishra* and Amitava Das*
- Analytical Chemistry , 2009
The chromogenic complex 1· Zn (where 1 is (E)-4-(4-
dimethylamino-phenylazo)-N,N-bispyridin-2-ylmethyl-benzenesulfonamide)
showed high affinity toward the phosphate
ion in tetrabutylammonium phosphate in acetonitrile
solution and could preferentially bind to adenosine triphosphate
(ATP) in aque...
The chromogenic complex 1· Zn (where 1 is (E)-4-(4-
dimethylamino-phenylazo)-N,N-bispyridin-2-ylmethyl-benzenesulfonamide)
showed high affinity toward the phosphate
ion in tetrabutylammonium phosphate in acetonitrile
solution and could preferentially bind to adenosine triphosphate
(ATP) in aqueous solution at physiological pH.
This binding caused a visual change in color, whereas no
such change was noticed with other related anions (adenosine
monophosphate, adenosine diphosphate, pyrophosphate,
and phosphate) of biological significance.
Thus, 1· Zn could be used as a staining agent for different
biological cells through binding to the ATP, generated in
situ by the mitochondria (in eukaryotes). For prokaryotes
(bacteria) the cell membrane takes care of the cells’ energy
conversion, since they lack mitochondria. ATP is produced
in their unique cell structure on the cell membrane,
which is not found in any eukaryotes. These stained cells
could be viewed with normal light microscopy. This
reagent could even be used for distinguishing the Grampositive
and the Gram-negative bacteria (prokaryotes).
This dye was found to be nonlipophilic in nature and
nontoxic to living microbes (eukaryotes and prokaryotes).
Further, stained cells were found to grow in their respective
media, and this confirmed the maintenance of viability
of the microbes even after staining, unlike with many other
dyes available commercially.