Найдено научных статей и публикаций: 1, для научной тематики: RNA virus replicationMembrane vesiclesVirus replication factoryEndoplasmic reticulum modification
1.
Соловьев А. Г. (ведущий научный сотрудник, лаборатория молекулярной биологии и биохимии, институт молекулярной медицины); Шария М.А. (профессор); Несвижский Юрий Владимирович (профессор)
, 2018
In infected plant cells, closterovirus replicative polyproteins 1a and 1ab drive membrane remodeling and formation of multivesicular replication platforms. Polyprotein 1a contains a variable Central Region (CR) between the methyltransferase and helicase domains. In a previous study, we have found th...
In infected plant cells, closterovirus replicative polyproteins 1a and 1ab drive membrane remodeling and formation of multivesicular replication platforms. Polyprotein 1a contains a variable Central Region (CR) between the methyltransferase and helicase domains. In a previous study, we have found that transient expression of the Beet yellows virus CR-2 segment (aa 1305-1494) in Nicotiana benthamiana induces the formation of ~1 µm mobile globules originating from the ER membranes. In the present study, sequence analysis has shown that a part of the CR named the “Zemlya region” (overlapping the CR-2), is conserved in all members of the Closterovirus genus and contains a predicted amphipathic helix (aa 1368-1385). By deletion analysis, the CR-2 region responsible for the induction of 1-μm globules has been mapped to aa 1368-1432. We suggest that the conserved membrane-modifying region of the BYV 1a may be involved in the biogenesis of closterovirus replication platforms.
