Purpose: to provide a method for detection of boluses defecation and urination spots in the study of the behavior of experimental biological objects in open arenas ethological tests with thermal registration. Material and methods. For identifying and quantifying defecation and urination biological objects using the compact thermal imaging camera FLIR SC 660 (FLIR Systems) with a wide-angle lens. Object of study is the laboratory rat. Morphometric parameters determined using image analysis software. Results. When used as a video recording device thermal imaging camera to get an objective, quantitative data on the level of defecation and urination in the study of behavior and emotional state of the experimental evaluation of warm-blooded biological objects in open arenas ethological tests. Recorded boluses defecation and urination spots in the study of the emotional component of the behavior of laboratory rats in the test apparatus «Open field», «Elevated plus maze», «Radial 8-Arm Maze» and «T-maze». Conclusion. Thermal video registration allows visualizing products vegetative urinary and fecal excretion in the dynamics of experimental studies in the evaluation of the psycho-emotional state of biological objects in various open arenas behavioral tests, carried out their detailed morphometric analysis and identify indicators of spatial distribution.