The paper describes a new method for the analysis of protein sequences - the method of analysis of the information structure (ANIS method). The method uses a new approach to describe amino acid sequences and identify hierarchically organized elements in the information structure of protein sequences. It was shown that the top-level information structure elements correspond to topologically stable elements of the three-dimensional structure (structural domains). A new approach for the identification of functionally important protein fragments was proposed based on the ANIS method. The approach was tested in the protein engineering experimental studies. Functionally important fragments of heat shock protein (hHSP70), human tumor necrosis factor (hTNF) and protein gp181 from phage φKZ were obtained. The proposed approach can be used for de novo protein design.
Institute of Physics Belgrade, 2014, SERBIA ISBN: 978-86-82441-40-3