The impact of integrins oscillation on intracellular calcium in conditions of native actin microfilaments network and under depolymerization was studied on the model of α(2)β(1)-integrins forced cyclic fluctuations with the use of integrin-specific magnetic microparticles, that were exposed to magnetic field after being cell-attached. It proved that α(2)β(1)-integrins are involved in the response of fibroblasts to mechanical deformation, activating depot-dependent calcium efflux in cytoplasm. Cytoskeletal actin microfilaments are also involved in the response via these receptors, which integrity is essential for normal functioning of the depot-dependent mechanism of Ca2+ efflux in cytoplasm of deformable fibroblasts.
Kot E.V., Kot Yu.G., Grytsenko M.A., Altukhova L.V., Persky Ye.E. Relation between integrins forced oscillation, degree of actin cytoskeleton polymerization and content of free Ca2+ of fibroblasts // European Researcher. – 2013. – Vol. 40. – № 2 – 1. – Р. 226 – 232.