Objective — To identify quantitative changes of the deuterium content, the intensity of free radical oxidation and antioxidant status of the blood system, as well as the effect of water with a modified isotope composition with low deuterium content on indicators of free radical oxidation of tissues in laboratory animals under physiological conditions and in inflammatory processes. Materials and methods — The object of the study was blood and homogenates of organs (liver, kidney) of male rats. The basis of the model of oxidative stress was a well-known model of wound healing proposed by L.A. Mamedov based on the surgical treatment of abscess model, and we performed its modification in the course of experimental studies. Determination of deuterium concentration in the plasma was carried out using nuclear magnetic resonance. The method of luminol-dependent H2O2-induced chemiluminescence was used for the detection of relatively unstable chemically active radicals in the plasma. Stable radicals detect by the EPR spectrometry. Results and Conclusion — Thus, it should be noted that blood plasma demonstrates reliable reduction in deuterium concentration when using deuterium depleted water that continues until the values of 90-100 ppm thereafter remaining practically unchanged. At the same time, deuterium depleted water affects prooxidant-antioxidant system of the body reducing the intensity of free radical oxidation and restoring the capacity of the endogenous antioxidant system.
Oxana Artsybasheva, Ekaterina Barysheva, Denis Shashkov, Roman Vlasov, Elena Tekutskaya Changes of oxidation during use the food diet with deuterium depleted water in laboratory animals with purulent inflammation // Russian Open Medical Journal, Vol. 3, Issue 2, 2014, pp. 201-0

Vinclozolin is one of the environmental contaminants, that has been shown to induce reproductive abnormalities in male rats. The aim of this study was to explore the adverse effects of vinclozolin on testicular oxidative stress in rats. Adult (100 day old) Wistar rats were injected inperitoneally with 1, 5 and 10 mg/kg bw vinclozolin on days 1, 7, 14 and 21 and maintained up to 50 days. The animals were killed by cervical dislocation at the end of maintainance period. Testes were isolated, cleared from adhering tissue/fluid and used to analyze oxidative status. A significant increase in the levels of lipid peroxidation was observed in the testis of vinclozolin exposed rats when compared with control rats. Furthermore, a significant decrease in the activity levels of antioxidant enzymes such as, superoxide dismutase, catalase was observed in the testis of vinclozolin treated rats when compared to control rats. The results were indicated that exposure to vinclozolin induces oxidative stress in the testis by increasing lipid peroxidation and decreasing the activities of antioxidant enzymes in rats. Our findings are consistent with a major role for accumulated free radical damage in the disruption of spermatogenic process in vinclozolin exposed rats thereby affects reproduction.

Vinclozolin (3-(3-5-dichlorophenyl)-5-methyl-oxazolidine-2,4-dione) is extensively used in the viticulture, agriculture and commercial horticulture to control several fungal pathogens and has been shown to induce reproductive abnormalities in male rats, causing reduced fertility. The mechanism of action of vinclozolin on male reproductive system is not clear. In the present study we investigated whether prenatal administration of vinclozolin induces oxidative stress in the testes and changes in sperm quality and quantity in epididymis in adult male rats. Pregnant rats were injected with 1, 5 and 10 mg vinclozolin/Kg body weight on 1st, 7th and 14th day of pregnancy and allowed to deliver. Male pups were maintained up to postnatal day 100 and used for assessing testicular and epididymal toxicity. Embryonic exposure to vinclozolin significantly decreased in the sperm density, number of motile sperm, viable sperm and HOS tail coiled sperm in adult rats. Significant increase in the levels of lipid peroxidation with a significant reduction in the activity levels of superoxide dismutase and catalase in testis was observed in rats exposed to vinclozolin during embryonic development. It can be concluded that prenatal exposure to vinclozolin induced oxidative stress in testis of adult rats thereby decreases the sperm quantity and deteriorates sperm quality.