Найдено научных статей и публикаций: 2, для научной тематики: Actin
1.
Kot E.V., Kot Yu.G., Grytsenko M.A., Altukhova L.V., Persky Ye.E.
, 2013
The impact of integrins oscillation on intracellular calcium in conditions of native
actin microfilaments network and under depolymerization was studied on the model of α(2)β(1)-integrins forced cyclic fluctuations with the use of integrin-specific magnetic microparticles, that
were exposed to mag...
The impact of integrins oscillation on intracellular calcium in conditions of native
actin microfilaments network and under depolymerization was studied on the model of α(2)β(1)-integrins forced cyclic fluctuations with the use of integrin-specific magnetic microparticles, that
were exposed to magnetic field after being cell-attached. It proved that α(2)β(1)-integrins are
involved in the response of fibroblasts to mechanical deformation, activating depot-dependent
calcium efflux in cytoplasm. Cytoskeletal actin microfilaments are also involved in the response via
these receptors, which integrity is essential for normal functioning of the depot-dependent
mechanism of Ca2+
efflux in cytoplasm of deformable fibroblasts.
Kot E.V., Kot Yu.G., Grytsenko M.A., Altukhova L.V., Persky Ye.E. Relation between integrins forced oscillation, degree of actin cytoskeleton polymerization and content of free Ca2+ of fibroblasts // European Researcher. – 2013. – Vol. 40. – № 2 – 1. – Р. 226 – 232.
2.
Matveev V.V.
- Physiol. Chem. Phys. & Med. NMR , 2000
Actin filaments are certainly believed to function as an intracellular signalling system; however, this is not confirmed by direct evidence. We used a two-layer actomyosin gel with a concen tration gradient of the troponin-tropomyosin complex (TT-complex, Ca2+-sensitive system) between the two layer...
Actin filaments are certainly believed to function as an intracellular signalling system; however, this is not confirmed by direct evidence. We used a two-layer actomyosin gel with a concen tration gradient of the troponin-tropomyosin complex (TT-complex, Ca2+-sensitive system) between the two layers. To prepare one layer of the system, natural actomyosin (nАМ) rich in TT-complex was used. To prepare the second layer, we used desensitized actomyosin (dAM) without the complex. All experimental studies were made in medium with a low ionic strength. Two phenomena were ob served: (1) dAM blocks Ca2+-sensitivity of nAM when the dAM weight portion in the system (as well as in mixed nAM+dAM suspension) reaches 40% and more; further increase of the dAM portion does not affect the Ca2+-sensitivity; (2) it was electrophoretically shown that a rapid diffusion of the TT-complex from nAM gel into the dAM gel took place. The apparent diffusion coefficient for the TT-complex in dAM gel is about (1-4)·10-4 cm2/sec, i.e. three orders higher than the same values for pro tein diffusion in water.
32: 167-178
