Synovial membrane derived mesenchymal stem cells (SM-MSCs) are known for their high proliferative and regenerative capacities, thereby presenting significant interest for applications in tissue engineering and regenerative medicine. The objective of this study was to evaluate the efficiency of hyaluronic acid (HA) supplement Ostenil® as a scaffold for non invasive autologous SM-MSC transplantation to regenerate the damaged cartilage in animal model.