The aim: to conduct analysis of the accumulation and biodistribution of gold nanoparticles in the structural and functional areas of mesenteric lymph nodes of white rats conducted with regard to the size and duration of the oral administration. The methods of dark field microscopy with Leica DM 2500 microscope were used to assess biodistribution of gold nanoparticle. The histochemical reaction of silver amplification with silver lactate by Danscher was used to confirm its identification. Results: It was found that the gold nanoparticles of 1-3 nm were not detected by these methods in any of the areas of the lymph nodes regardless of the duration of administration, gold nanoparticles of sizes 15 and 50 nm were detected in the form of clusters in the cytoplasm of macrophages and lymphocytes mainly in the mantle zone of lymphoid follicles and brain strands. Conclusion: It was established that the gold nanoparticles of sizes 15 and 50 nm were detected in the form of clusters in the cytoplasm of macrophages and lymphocytes
Olga V. Zlobina, Svetlana S. Pakhomiy, Alla B. Bucharskaya, Irina O. Bugaeva, Galina N. Maslyakova, Nikolai G. Khlebtsov, Boris N. Khlebtsov, Vladimir A. Bogatyrev Accumulation and biodistribution of gold nanoparticles in the mesenteric lymph nodes at oral administration // Russian Open Medical Journal, Vol. 2, Issue 3, 2013, pp. 301-0

The influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the morphokinetics of mesenterial lymphatic node cell populations of healthy laboratory animals was investigated. It was established that the oral administration of gold nanoparticles caused the changes of morphokinetics of mesenterial lymphatic node cell populations. The morphological reorganizations in the mesenterial lymphatic nodes testified about activation of migration processes, the proliferation and differentiation processes of immunocompetent cells, which assumes the presence of immunomodulating action of gold nanoparticles.
O.V. Zlobina, Irina O. Bugaeva, Galina N. Maslyakova, S.S. Firsova, Alla B. Bucharskaya, N.G. Khlebtsov, B.N. Khlebtsov, L.A. Dykman Morphokinetics of mesenterial lymphatic node cell populations at exposure of gold nanoparticles in experiment // Russian Open Medical Journal, Vol. 1, Issue 3, 2012, pp. 302-0

The aim of the article is to conduct analysis of accumulation and biodistribution of gold nanoparticles in the structural and functional areas of mesenteric lymph nodes of white rats. Particular attention is paid to the size and duration of oral administration. Material and Methods: Dark field microscopy with Leica DM 2500 microscope has been used to assess biodistribution of gold nanoparticle. The histochemical reaction of silver amplification with silver lactate by Danscher has been used to confirm gold nanoparticles identification. Results: It has been found that regardless of the duration of administration gold nanoparticles of 1-3 nm have not been detected by these methods in the lymph nodes. Gold nanoparticles of 15 and 50 nm have been found out in the form of clusters in the cytoplasm of macrophages and lymphocytes mainly in the mantle zone of lymphoid follicles and brain strands. Conclusion: It has been established that the gold nanoparticles of 15 and 50 nm has been detected in the form of clusters in the cytoplasm of macrophages and lymphocytes.
Zlobina O.V., Pakhomiy S. S., Bucharskaya A.B., Bugaeva I.O., Maslyakova G.N., Khlebtsov N.G., Khlebtsov B.N., Bogatyrev V.A. Analysis of accumulation and biodistribution of gold nanoparticles in mesenteric lymph nodes by oral administration // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 1, 2013, pp. 17-20

The research goal is to investigate the influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the morphokinetics of mesenterial lymphatic node cell populations of healthy laboratory animals. Experiment included 24 white rats. The investigation was conducted in 4 groups of animals. The animals were administered the gold nanoparticles orally for 15 days. It was established that the oral administration of gold nanoparticles caused the changes of morphokinetics of mesenterial lymphatic node cell populations. The morphological alterations in the mesenterial lymphatic nodes assisted in activation of migration processes, the proliferation and differentiation. The immu-nomodulatory action of gold nanoparticles was proved
Zlobina O.V., Bugaeva I. О., Maslyakova G.N., Firsova S.S., Bucharskaya А.В., Khlebtsov N.G., Khlebtsov B.N., Dykman L.A. Morphokinetics of mesenterial lymphatic node cell populations in exposure of gold nanoparticles within experimental work // Saratov Journal of Medical Scientific Research, Vol. 7, Issue 2, 2011, pp. 353-357