The aim: to conduct analysis of the accumulation and biodistribution of gold nanoparticles in the structural and functional areas of mesenteric lymph nodes of white rats conducted with regard to the size and duration of the oral administration. The methods of dark field microscopy with Leica DM 2500 microscope were used to assess biodistribution of gold nanoparticle. The histochemical reaction of silver amplification with silver lactate by Danscher was used to confirm its identification. Results: It was found that the gold nanoparticles of 1-3 nm were not detected by these methods in any of the areas of the lymph nodes regardless of the duration of administration, gold nanoparticles of sizes 15 and 50 nm were detected in the form of clusters in the cytoplasm of macrophages and lymphocytes mainly in the mantle zone of lymphoid follicles and brain strands. Conclusion: It was established that the gold nanoparticles of sizes 15 and 50 nm were detected in the form of clusters in the cytoplasm of macrophages and lymphocytes
Olga V. Zlobina, Svetlana S. Pakhomiy, Alla B. Bucharskaya, Irina O. Bugaeva, Galina N. Maslyakova, Nikolai G. Khlebtsov, Boris N. Khlebtsov, Vladimir A. Bogatyrev Accumulation and biodistribution of gold nanoparticles in the mesenteric lymph nodes at oral administration // Russian Open Medical Journal, Vol. 2, Issue 3, 2013, pp. 301-0

The influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the morphokinetics of mesenterial lymphatic node cell populations of healthy laboratory animals was investigated. It was established that the oral administration of gold nanoparticles caused the changes of morphokinetics of mesenterial lymphatic node cell populations. The morphological reorganizations in the mesenterial lymphatic nodes testified about activation of migration processes, the proliferation and differentiation processes of immunocompetent cells, which assumes the presence of immunomodulating action of gold nanoparticles.
O.V. Zlobina, Irina O. Bugaeva, Galina N. Maslyakova, S.S. Firsova, Alla B. Bucharskaya, N.G. Khlebtsov, B.N. Khlebtsov, L.A. Dykman Morphokinetics of mesenterial lymphatic node cell populations at exposure of gold nanoparticles in experiment // Russian Open Medical Journal, Vol. 1, Issue 3, 2012, pp. 302-0

Aim: to evaluate the severity of morphological changes in the liver of laboratory animals with prolonged intake of pegilated gold nanoparticles of different sizes from 2 to 50 nm. Material and methods. The experiment was performed on 240 albino rats divided into 4 groups. The animals of experimental groups were orally administered the gold nanoparticles in a specific pattern. Results. Morphological changes in liver depend on the particle size and the duration of administration. Developing pathological changes in the liver are reversible, as evidenced by the gradual recovery of the liver structure 14 days after administration. Conclusion. The most significant changes in the liver were found in the experimental groups with a 30-day administration of 2 and 50 nm gold nanoparticles that show the size-dependent nature of their impact.
Pakhomy S.S., Bucharskaya А.В., Navolokin N.A., Maslyakova G.N., Zlobina O.V., Khlebtsov N.G., Khlebtsov B.N., Bogatyrev V.A. Morphological changes in liver of laboratory animals at prolonged intake of gold nanoparticles // Saratov Journal of Medical Scientific Research, Vol. 10, Issue 2, 2014, pp. 250-256

Laser thermal therapy is increasingly being used in clinical practice for cancer treatment. Its major limitation is associated with low spatial selectivity. This restriction may be excluded by the use of plasmon-resonant gold nanoparticles as photothermal sensitizers. Tunable synthesis, unique optical properties and low toxicity make gold nanostructures promising as a therapeutic agent for cancer treatment. The research work has presented the experimental data that concern the use of plasmon photothermal therapy for the treatment of transplanted and induced tumors of laboratory animals. Taking into consideration the research results it is determined that the transition of this therapeutic technology into the clinical practice can be predicted.
Chumakov D.S., Basko M.V., Dikht N.I., Bucharskaya A.B., Rodionova Т.I., Terentyuk G.S. Laser thermal therapy is increasingly being used in clinical practice for cancer treatment. Its major limitation is associated with low spatial selectivity. This restriction may be excluded by the use of plasmon-resonant gold nanoparticles as photothermal // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 4, 2013, pp. 700-706

The purpose of the article is to assess the severity of the morphological changes in the internal organs of laboratory animals with prolonged oral administration of gold nanoparticles of different sizes. Material and methods: The experiment was carried out on 40 albino rats which were divided into 4 groups. Animals of the experimental group received oral gold nanoparticles by the appropriate scheme. Results: At comparing the morphological changes in the internal organs of laboratory animals it was established that the introduction of gold nanoparticles of different sizes leads to the same type of disturbances of morphological structure, the gold nanoparticles of 1-3 and 50 nm cause the most expressive changes. Conclusion: The most significant morphological modifications in the internal organs were caused by gold nanoparticles of 1-3 and 50 nm. It points out to the size-dependence of the effect of gold nanoparticles.
Maslyakova G.N., Pakhomiy S. S., Bucharskaya А.В., Zlobina O.V., Navolokin N.A., Ponukalin A.N., Khlebtsov N.B., Khlebtsov B.N., Bogatyrev V.A. Morphological changes in the organs of laboratory animals during the prolonged oral administration of gold nanoparticles // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 2, 2013, pp. 208-213

The aim of the article is to conduct analysis of accumulation and biodistribution of gold nanoparticles in the structural and functional areas of mesenteric lymph nodes of white rats. Particular attention is paid to the size and duration of oral administration. Material and Methods: Dark field microscopy with Leica DM 2500 microscope has been used to assess biodistribution of gold nanoparticle. The histochemical reaction of silver amplification with silver lactate by Danscher has been used to confirm gold nanoparticles identification. Results: It has been found that regardless of the duration of administration gold nanoparticles of 1-3 nm have not been detected by these methods in the lymph nodes. Gold nanoparticles of 15 and 50 nm have been found out in the form of clusters in the cytoplasm of macrophages and lymphocytes mainly in the mantle zone of lymphoid follicles and brain strands. Conclusion: It has been established that the gold nanoparticles of 15 and 50 nm has been detected in the form of clusters in the cytoplasm of macrophages and lymphocytes.
Zlobina O.V., Pakhomiy S. S., Bucharskaya A.B., Bugaeva I.O., Maslyakova G.N., Khlebtsov N.G., Khlebtsov B.N., Bogatyrev V.A. Analysis of accumulation and biodistribution of gold nanoparticles in mesenteric lymph nodes by oral administration // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 1, 2013, pp. 17-20

The research goal is to investigate the influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the morphokinetics of mesenterial lymphatic node cell populations of healthy laboratory animals. Experiment included 24 white rats. The investigation was conducted in 4 groups of animals. The animals were administered the gold nanoparticles orally for 15 days. It was established that the oral administration of gold nanoparticles caused the changes of morphokinetics of mesenterial lymphatic node cell populations. The morphological alterations in the mesenterial lymphatic nodes assisted in activation of migration processes, the proliferation and differentiation. The immu-nomodulatory action of gold nanoparticles was proved
Zlobina O.V., Bugaeva I. О., Maslyakova G.N., Firsova S.S., Bucharskaya А.В., Khlebtsov N.G., Khlebtsov B.N., Dykman L.A. Morphokinetics of mesenterial lymphatic node cell populations in exposure of gold nanoparticles within experimental work // Saratov Journal of Medical Scientific Research, Vol. 7, Issue 2, 2011, pp. 353-357