Найдено научных статей и публикаций: 2, для научной тематики: Chromatography
1.
Bychkov E.N., Serkova S.A., Arsentieva L.A., Frantsuzova T.S., Bluvshtein G.A., Borodulin V.B.
- Saratov Journal of Medical Scientific Research , 2011
The goal of the present paper was to find effective biochemical markers of chronic narcotization. Materials and methods. The results of biochemical research of biological liquids in human organism and chromatographic methods of drug substances identification in cadaveric urine were analyzed. Researc...
The goal of the present paper was to find effective biochemical markers of chronic narcotization. Materials and methods. The results of biochemical research of biological liquids in human organism and chromatographic methods of drug substances identification in cadaveric urine were analyzed. Research of 197 samples of different biologic liquids of 10 persons was carried out. Control was fulfilled on the basis of 135 samples of biological liquids took of 12 persons. Methods of identification of drug substances are presented, chromatographic and spectral characteristics of drug substances are described. Interrelations of biochemical indices at positive study of drug substances identification were conducted. The increased urea metabolic concentration in cadaveric blood of drug addicts was revealed in comparison with control specimens. Results. (Numeral results are given in Table 3). Correct correlation of biochemical markers was not established. Conclusion. The present results suppose continued study of informative reliable biochemical markers of chronic drug intoxication
Bychkov E.N., Serkova S.A., Arsentieva L.A., Frantsuzova T.S., Bluvshtein G.A., Borodulin V.B. General methods of identification of narcotic substances in cadaveric urine and biochemical indices in blood at positive identification of drug substances // Saratov Journal of Medical Scientific Research, Vol. 7, Issue 3, 2011, pp. 642-646
2.
S. K. Mishra, A. Shrivastav, S. Mishra.
- Protein Expression and Purification , 2011
C-phycoerythrin was isolated and purified from marine Pseudanabaena sp. using two step chromatographic methods. Phycobiliproteins in the marine Pseudanabaena was extracted in 100mM phosphate buffer (pH 7.2) and precipitated by salting out. The precipitated C-phycoerythrin was purified by gel filtrat...
C-phycoerythrin was isolated and purified from marine Pseudanabaena sp. using two step chromatographic methods. Phycobiliproteins in the marine Pseudanabaena was extracted in 100mM phosphate buffer (pH 7.2) and precipitated by salting out. The precipitated C-phycoerythrin was purified by gel filtration with Sephadex G-150, and then it was purified by ion exchange chromatography on DEAE cellulose, which was developed by linear ionic strength gradients. Purified phycoerythrin showed absorption maxima at 568 and 541nm, and displayed a fluorescence maximum at 578nm. The absorbance ratio A(568)/A(280), a criterion for purity (purity ratio) achieved was 6.86. It showed a single band on examination by polyacrylamide gel electrophoresis (PAGE). The polypeptide analysis of the purified C-phycoerythrin by SDS-PAGE demonstrated that it contained two chromophore-carrying subunits. The yield of purified C-phycoerythrin obtained was 13.6mg/g of the cell dry weight with 47% of yield. Obtaining highly pure C-phycoerythrin allows one to evaluate its fluorescence properties for future applications in biochemical and biomedical research.