http://scipeople.ru/publication/118623/; Toxicity of Gold Nanoparticles for Plants in Experimental Aquatic System S. A. Ostroumov, V. A. Poklonov, S. V. Kotelevtsev, and S. N. Orlov Department of Biology, Moscow State University, Moscow, Russia email: ostroumov@mail.bio.msu.ru Received April 10, 2013 Abstract: Increased production and use of nanomaterials can lead to new types of pollution of the environment, including aquatic ecosystems. Pollution of the aqueous environment with nanoparticles can be a new type of pollution of the environment. This requires a more detailed study of the biological effects during exposure of nanoparticles on aquatic organisms. The interactions of gold nanoparticles (Au) with aquatic macrophytes Ceratophyllum demersum have been studied. Aquatic microcosms with these plants were used. Gold nanoparticles (Au) were added to the aqueous medium of C. demersum macrophyte containing microcosms. The state of the plants was then analyzed. Phytotoxicity of Au nanoparticles for aquatic macrophytes was shown for the first time. A new method of phytotoxicity detection was suggested and successfully approved. Phytotoxicity at a concentration of Au (in the form of nanoparticles) of 6 . 10–6 M–1.8 . 10–5 M was shown. Original Russian Text © S.A. Ostroumov, V.A. Poklonov, S.V. Kotelevtsev, S.N. Orlov, 2014, published in Vestnik Moskovskogo Universiteta. Biologiya, 2014, No. 3, pp. 19–23. Keywords: hydrobionts, aquatic, microcosms, macrophytes, nanoparticles, Au, Ceratophyllum demersum, gold, toxicity, phytotest, phytotoxicity, nanomaterials, nanotoxicity, nanotechnology DOI: 10.3103/S0096392514030080
ISSN 0096-3925, Moscow University Biological Sciences Bulletin, 2014, Vol. 69, No. 3, pp. 108–112. © Allerton Press, Inc., 2014.

Objective: Morphological changes of internal organs and brain of laboratory animals have been studied at prolonged oral administration of iron nanoparticles in experiments. Materials and methods: Iron nanoparticles 70±10 nm, 28 male white rats, and morphological methods of evaluation have been used in the experiment. Results: Studies have shown that the changes depend on nanoparticle concentration and signs of circulatory disorders and degenerative changes of parenchymatous elements
Navolokin N.A., Kun S.M. Morphological changes of internal organs and brain at prolonged oral administration of iron nanoparticles // Saratov Journal of Medical Scientific Research, Vol. 7, Issue 4, 2011, pp. 760-762

Ostroumov S.A., Kotelevtsev S.V. Toxicology of nanomaterials and environment. - Ecologica. 2011, vol. 18, issue 61, pp. 3-10. Manufactured nanoparticles demonstrated a variety of biological activities, including toxicity. The goal of this review articles is to summarize some evidence of a variety of toxic effects produced by manufactured nanoparticles, including both the data from literature and the new data of the authors (e.g., [42]). The toxicity of nanomaterials was shown both to prokaryotic and eukaryotic organisms. As for eukaryotic organisms, toxicity was found in bioassays with both animal and plant test systems. In further studies, it is necessary to continue the studies of various aspects of toxicity of nanoparticles, and to extend the range of organisms and test systems that are being used for assessing the biological effects of nanomaterials. The methods that were previously developed to study phytotoxicity of chemicals [15-28, 48] will be useful to generate new data on toxicology of nanomaterials.
Ecologica. 2011, vol. 18, issue 61, pp. 3-10.