Cardiomyopathies (CMP) are considered diseases with unknown etiology. CMP are usually diagnosed on the late stages of the disease. It is an important task to seek methods which allow diagnosing CMP on the early stages of development. Aim – to estimate the type of polymorphic variants of angiotensin converting enzyme (ACE), glutathione S-transferase (GSTM1) frequency distribution among the CMP sick and healthy individuals living in the Republic of Bashkortostan, and to substantiate their role in the CMP pathogenesis. Material and Methods – There were 67 CMP sick put under observation. Out of them40 werediagnosed ischemic cardiomyopathy (ICM), and 27 were diagnosed dilated cardiomyopathy (DCM). The control group was made up of 110 practically healthy people. The results.In the ICM sick group the DD genotype (χ2=4.8; p=0.029) was definitely found more frequently, also in case of DCM the increase of DD genotype (χ2=4.0, p=0.044) frequency was ascertained. Conclusion – The genetic analysis of polymorphic locus of I/D ACE gene in the ICM and DCM sick showed decrease in frequency of occurrence II, ID genotypes, I allele and increase of D genotype and D allele which points out the increased risk of these cardiovascular diseases.
Arvid R. Berg, A.N. Chepurnaya, D.O. Karimov, T.V. Viktorova, V.I. Nikulicheva, G.Sh. Safuanova Analysis of gene polymorphic variants of angiotensinconverting enzyme, glutathione S-transferase in cardiomyopathy sick patients // Russian Open Medical Journal, Vol. 1, Issue 3, 2012, pp. 301-0

im. The human GSTP1 is a major enzyme of phase II detoxification in the most cell types. Aberrant expression of GSTP1 is associated with carcinogenesis and development of multidrug resistance. The GSTP1 gene expression is regulated at multiple levels including transcriptional, post-transcriptional and post-translational. We concentrated our attention on the transcriptional level of regulation. Methods. Transient transfection of Me45 melanoma cells with constructs containing the luciferase gene under the control of complete and truncated GSTP1 promoter was utilized to identify a role of different promoter regions in regulation of the gene transcription in Me45 cells. To identify the transcription factors, interacting with the GSTP1 promoter sites, the competitive EMSA and super shift assay were applied. Results. GSTP1 transcription in Me45 cells is positively regulated by binding NF-kB to the cognate site and ERb in complex with unknown protein to the ARE site; the complex of ERb with c-Fos negatively regulates the gene expression via CRE site. The interaction of c-Fos/ERb with GSTP1 CRE site and indirect interaction of ERb with GSTP1 ARE were identified. Conclusions. The positive regulation of the human GSTP1 gene in Me45 melanoma cells is exerted via NF-kB and ARE sites and the negative one via CRE site of the promoter. ERb is indirectly involved in the regulation of GSTP1 transcription. It is bound via c-Fos with CRE site and via unknown protein with ARE site.
Crosstalk between transcription factors in regulation of the human glutathione S-transferase P1 gene expression in Me45 melanoma cells / А.М. Slonchak, А. Cwieduk, J. Rzerzowska-Wolny, M.Yu. Obolenskaya // Біополімери і клітина. — 2009. — Т. 25, № 3. — С. 210–217. — Бібліогр.: 22 назв. — англ.