Найдено научных статей и публикаций: 3, для научной тематики: ANIS method
1.
A. A. Anashkina, A. N. Nekrasov
- Russian Journal of Numerical Analysis and Mathematical Modelling , 2014
The paper describes a new method of Information Structure Analysis (ISAN) for protein
sequences. The method uses a new approximation for description of protein sequences, which allows
us to detect hierarchically organized elements (Information Structure Elements (ISEL)) in them. It
is shown that ele...
The paper describes a new method of Information Structure Analysis (ISAN) for protein
sequences. The method uses a new approximation for description of protein sequences, which allows
us to detect hierarchically organized elements (Information Structure Elements (ISEL)) in them. It
is shown that elements of the higher hierarchy level of information structure correspond to stable
elements in the spatial structure of globular proteins. Based on the ISAN method, we propose an approach
to design of the new sequences of recombinant proteins. The proposed approach was confirmed
experimentally in the case of obtaining functionally active recombinant proteins.
Russian Journal of Numerical Analysis and Mathematical Modelling (2014) vol. 29(5), pp. 265–273
2.
A.N. Nekrasov, L.E. Petrovskaya, V.A. Toporova, E.A. Kryukova, A.V. Rodina, E.Yu. Moskaleva, M.P. Kirpichnikov
- Biochemistry (Moscow) , 2009
Interleukin13 (IL13) is one of the cytokines involved in the development of Th2type immune response. It plays an important role in the pathogenesis of asthma and other allergic diseases. Two deletion forms of IL13 were constructed on a basis of informational structure analysis and expressed in E...
Interleukin13 (IL13) is one of the cytokines involved in the development of Th2type immune response. It plays an important role in the pathogenesis of asthma and other allergic diseases. Two deletion forms of IL13 were constructed on a basis of informational structure analysis and expressed in E. coli cells. They were found to differ in ability to stimulate proliferation of TF1 cell line. Deletion variant 146 (DV146) completely lacks such activity, whereas DV148 provides about 50% of the proliferation stimulation. The simultaneous addition of DV146 with fulllength IL13 suppresses proliferation depending on the concentration of the deletion form. Thus, the designed protein acts as an antagonist of IL13.
Biochemistry (Moscow), 2009, Vol. 74, No. 4, pp. 399-405.
3.
T. V. Ostroverkhova, R. V. Chertkova, A. N. Nekrasov, D. A. Dolgikh, M. P. Kirpichnikov
- Moscow University Biological Sciences Bulletin , 2011
Informational structure of cytochrome c was investigated using the ANIS method (Analysis of
Informational Structure Method). Mutant variants of cytochrome c gene were constructed on the basis of data from the ANIS method. The mutations carry substitutions reducing electrontransport activity of cyto...
Informational structure of cytochrome c was investigated using the ANIS method (Analysis of
Informational Structure Method). Mutant variants of cytochrome c gene were constructed on the basis of data from the ANIS method. The mutations carry substitutions reducing electrontransport activity of cytochrome c in the mitochondrial respiratory chain. These mutant genes were obtained and expressed in the bacterial system. The biological activity of the obtained cytochrome c mutant variants interacting with complexes III and IV of the respiratory chain in the system of rat liver mitoplasts.
Moscow University Biological Sciences Bulletin, 2011, Vol. 66, No. 2, pp. 65–67.
