10 most cited publications authored and co-authored by S.A.Ostroumov. Among the ten publications, the top three most cited publications (No. 1-3) were cited over 300 times (Date: April 11, 2010) 1. L. A. Drachev, A. A. Jasaitis, A. D. Kaulen, A. A. Kondrashin, E. A. Liberman, I. B. Nemecek, S. A. Ostroumov, A. Yu. Semenov, V. P. Skulachev Direct measurement of electric current generation by cytochrome oxidase, H+-ATPase and bacteriorhodopsin. – Nature, 249, 321 - 324 (24 May 1974); doi:10.1038/249321a0 One of key publications toward the proof of the chemiosmotic hypothesis of P. Mitchell [Mitchell, P., Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation, Glynn Research, (Bodmin, 1966); Chemiosmotic Coupling and Energy Transduction, Glynn Research (Bodmin, 1968)]. After the hypothesis was proved, he (P.Mitchell) was awarded the Nobel Prize. --- 2. Reconstitution of biological molecular generators of electric current. Bacteriorhodopsin // J. Biol. Chem. 1976, vol. 251, p.7059-7065. Abstract in English. [Co-authors / Совместно: Drachev L.A., Frolov V.N., Kaulen A.D., Liberman E.A., S.A.O., Plakunova V.G., Semenov A.Y., Skulachev V.P.] Abstract: 1. Photoinduced generation of electric current by bacteriorhodopsin, incorporated into the planar phospholipid membrane, has been directly measured with conventional electrometer techniques. 2. Two methods for bacteriorhodopsin incorporation have been developed: (a) formation of planar membrane from a mixture of decane solution of phospholipids and of the fraction of violet fragments of the Halobacterium halobium membrane (bacteriorhodopsin sheets), and (b) adhesion of bacteriorhodopsin-containing reconstituted spherical membranes (proteoliposomes) to the planar membrane in the presence of Ca2+ or some other cations. In both cases, illumination was found to induce electric current generation directed across the planar membrane, an effect which was measured by macroelectrodes immersed into electrolyte solutions on both sides of the membrane. As the previous paper in this list, another key step to the proof of the Mitchell’s hypothesis. P.Mitchell was finally warded the Nobel Prize. --- 3. Electrogenesis by bacteriorhodopsin incorporated in a planar phospholipid membrane. L A Drachev, A D Kaulen, S A Ostroumov, V P Skulachev FEBS Letters 1974; 39(1):43-45 ICID: 894353; Article type: Original article; PMID 4851814 to show this article in PubMed database; 4. Book: Conservation of living nature and resources: problems, trends, and prospects. A.V. Yablokov, S.A. Ostroumov. 1991. Publisher: Springer-Verlag Abstract: The deterioration of the environment has become one of the most serious problems of the twentieth century. The 12 chapters in this book: (1) the dangerous extent of human impact on the biosphere; (2) molecular genetic level; (3) problems at the Ontogenetic level; (4) problems at the species-population level; (5) problems at the ecosystem level; (6) economic problems; (7) organizational and legal problems of the protection of living nature; (8) problems of prognostication; (9) protection of living nature and its connection with other global problems; (10) need for ecologization of society's activities; (11) ways of preserving living nature; and (12) scientific foundations and theory of living nature protection within the system of biological science, analyse the problems of conservation and suggest ways of helping to solve them. --- 5. Inhibitory analysis of top-down control: new keys to studying eutrophication, algal blooms, and water self-purification // Hydrobiologia. 2002. vol. 469. P.117-129. 8 tables. Bibliogr. 71 refs. [Top-down control is an important type of interspecies interactions in food webs. It is especially important for aquatic ecosystems. Phytoplankton grazers contribute to the top-down control of phytoplankton populations. This article is focused on the role of benthic suspension-feeders (filter - feeders) in the control of plankton populations as a result of water filtering and the removal of suspended matter (including the cells of plankton) from the water column. New data on the inhibitory effects of synthetic surfactants and detergents on benthic filter-feeders (freshwater mussels Unio tumidus, U. pictorum, marine mussels Mytilus galloprovincialis, M. edulis, and oysters Crassostrea gigas) are presented and discussed. Importance and efficiency of that approach to the problems of eutrophication and water self-purification is pointed out. The chemical pollution may pose a threat to the natural top-down control of phytoplankton and water self-purification processes. The latter are an important prerequisite for sustainable use of aquatic resources. Surfactant SDS at a concentration of 0.5 mg/L inhibited water filtration by Crassostrea gigas, at a concentration of 1.7 mg/L suppressed the filtering activity of M. galloprovincialis, at that of 1-5 mg/L decreased water filtration by M. edulis. The non-ionic surfactant Triton X-100 (TX100) at a concentration of 0.5-5 mg/L induced a sharp slowdown in water filtration by M. edulis, at 1 and 5 mg/L it caused a decrease in the filtration rate by the freshwater bivalve Unio tumidus. The cationic surfactant TDTMA at 1-2 mg/L constrained the filtering activity of the freshwater bivalve Unio pictorum. 4 detergents (6.7-50 mg/L) hindered the filtration by M. galloprovincialis. 3 detergents (1-30 mg/L) slowed down the clearance rate during water filtration by Crassostrea gigas. The shampoo AHC (Avon Herbal Care) at concentrations 5-60 mg/L arrested the filtration by M. galloprovincialis. A step toward modernization of the contemporary understanding of how ecosystem functions and how it performs useful ecosystem services] (Springer Press, Dordrecht). ISSN 0018-8158 (Print) 1573-5117 (Online) DOI 10.1023/A:1015559123646; http://www.moipros.ru/files/author_4_article_9.doc; www.springerlink.com/index/R9PTJEQ5FK8VLA6M.pdf; http://scipeople.com/uploads/materials/4389/2Hydrobiologia469p117w%20Addendum.DOC --- 6. Polyfunctional role of biodiversity in processes leading to water purification: current conceptualizations and concluding remarks // Hydrobiologia. 2002. v. 469 (1-3): P.203-204. Bibliogr. 8 refs. ISSN 0018-8158 (Print) 1573-5117 (Online). [Selected elements of a new vision of the role of biodiversity in water purification and maintaining the natural purification potential of ecosystems, elements of the theory of water self-purification. Some fundamental principles that characterize the pivotal roles of the biodiversity of filter-feeders in ecosystems. Among those roles are: (1) the role of ecological repair of water quality, (2) the role of contributing to reliability and stability of the functioning of the ecosystem, (3) the role of contributing to creation of habitat heterogeneity, (4) the role of contributing to acceleration of migration of chemical elements. It is an important feature of the biomachinery of filter-feeders that it removes from water various particles of a very broad range of sizes. Another important principle is that the amount of the organic matter filtered out of water is larger than the amount assimilated so that a significant part of the removed material serves no useful function to the organism of the filter-feeder, but serves a beneficial function to some other species and to the ecosystem as a whole. The new experiments by the author additionally demonstrated a vulnerability of the filtration activity of filter feeders (e.g., bivalves and rotifers) to some xenobiotics (tetradecyltrymethylammonium bromide, heavy metals and some others). The inhibition of the filtration activity of filter-feeders may lead to the situation previously described as that of an ecological impairment of the second type]. DOI 10.1007/s10750-004-1875-1. http://scipeople.com/uploads/materials/4389/2H469p203.Polyfunctional.role.w.Addendum.rtf --- 7. Biological filtering and ecological machinery for self-purification and bioremediation in aquatic ecosystems: towards a holistic view // Rivista di Biologia / Biology Forum. 1998. 91: 247-258. According to one of the approaches to the definition of criteria for the phenomenon of life, the key attribute is the ability of the system for some self-regulating and self-supporting. Part of such holistic functions of aquatic ecosystems as self-regulating and self-supporting is their cleaning the water via a multitude of various mechanisms. The goal of this paper is to present some fundamental elements of the theory of ecosystem self-purification which emphasizes the importance of the four functional biological filters that are instrumental in purification and upgrading the quality of water in aquatic ecosystems. These functional filters are: (1) direct water filtering by aquatic organisms that are filter-feeders; (2) the filter (represented mainly by communities of aquatic plants/periphyton) which prevents input of pollutants and biogenic elements (N, P) from land into water bodies; (3) the filter (represented by benthic organisms) which prevents re-entry of pollutants and biogenic elements from the bottom sediments into the water; (4) the filter (represented by microorganisms attached to particles which are suspended in the water) that provides microbiological treatment of water column. New experimental data by the author reveal the role of man-made effects on the ecological machinery which purifies water. The analysis and discussion lead to the holistic theory of the natural process of bioremediation of aquatic ecosystems. [An innovative analysis of the role of organisms in water filtration in ecosystems. Water filtration is a key component of ecological biomachinery for maintaining the natural purification potential of ecosystems. Among new facts: the synthetic surfactant SDS 1 mg/L inhibited filtration (35-95 min, 16°С) of bivalve Mytilus edulis and the removal of the cells of algae Isochrysis galbana from water. Amount of cells that stayed in water unfiltered after 95 min, was 3 times as much as the amount of cells in the control without SDS]. Abstracts in English and Italian. http://www.ncbi.nlm.nih.gov/pubmed/9857844?dopt=Abstract; PMID: 9857844 [PubMed - indexed for MEDLINE]; http://scipeople.com/publication/69542/; --- 8. E.L. Barsky1, 2, E.A. Bonch-Osmolovskaya1, 2, S.A. Ostroumov1, 2, V.D. Samuilov1, 2 and V.P. Skulachev1, 2 A study on the membrane potential and pH gradient in chromatophores and intact cells of photosynthetic bacteria. - Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume 387, Issue 2, 15 May 1975, Pages 388-395. doi:10.1016/0005-2728(75)90118-8 1Department of Bioenergetics, Laboratory of Bioorganic Chemistry, Moscow 117234 2Department of Microbiology, Biological Faculty, Moscow State University, Moscow 117234 Abstract. Generation of membrane potential (Δψ) and transmembrane pH difference (ΔpH) was studied in PPi-energized chromatophores of Rhodospirillum rubrum by means of measurements of carotenoid and bacteriochlorophyll absorption changes, atebrin and 8-anilinonaphthalene-1-sulphonate fluorescence responses, and phenyldicarbaundecaborane transport. The data obtained are consistent with the suggestion that carotenoid, bacteriochlorophyll and phenyldicarbaundecaborane responses are indicators of Δψ, while an atebrin response is an indicator of ΔpH. The fluorescence of 8-anilinonaphthalene-1-sulphonate is affected both by Δψ and ΔpH. Abbreviations: ANS−, 8-anilinonaphthalene-1-sulfonate; CCCP, 2,4,6-trichlorocarbonyl cyanide phenylhydrazone; PCB−, phenyldicarbaundecaborane; PPi, inorganic pyrophosphate --- 9. On the biotic self-purification of aquatic ecosystems: elements of the theory. - DAN (Doklady Akademii Nauk), Vol. 396, 2004, No. 1, p. 136–141. [System of elements of the theory of biotic maintaining the natural purification potential of ecosystems]. The paper was awarded the honorary Diploma from the Academy of Water Sciences (2006). In Russ., translated into Eng.: On the biotic self-purification of aquatic ecosystems: elements of the theory. - Doklady Biological Sciences, V. 396, 2004, p. 206–211. (Translated from DAN, V.396, No.1, 2004, p.136–141). Basic elements are formulated for a qualitative theory of the polyfunctional role of the biota in improving water quality and doing self-purification of water in aquatic ecosystems. The elements of the theory covers the following: sources of energy for the mechanisms of water self-purification; the main functional blocks of the system of self-purification; the system of the main processes that are involved; the analysis of the degree of participation of the main large taxa; the reliability of the mechanisms of water self-purification; regulation of the processes; the response of the mechanisms of water self-purification towards the external influences (man-made impacts, pollution); and some conclusions relevant to the practice of environment protection. In support of the theory, the results are given of the author's experiments which demonstrated the ability of some pollutants (surfactants, detergents, and some others) to inhibit the water filtration activity of marine filter-feeders, namely, the bivalve mollusks Mytilus galloprovincialis, Mytilus edulis, and Crassostrea gigas. ISSN 0012-4966 (Print) 1608-3105 (Online). Distributed by Springer, orderdept@springer-sbm.com. DOI: 10.1023/B:DOBS.0000033278.12858.12; http://scipeople.ru/users/2943391/; https://www.researchgate.net/file.FileLoader.html?key=60f338228d6f3c5114d223ab81e15d3b; http://www.springerlink.com/content/t0nv6rk522230175/; - - - - - 10. [book] Introduction to Biochemical Ecology. 1986. Moscow. Moscow University Press. 176 p. Figures, tables. Bibliogr.: pp. 168-174. [Analyzed: Ecological roles of natural and man-made chemicals. Inter alia, some problems of chemical communication and regulation in natural populations and ecosystems, environmental toxicology and chemistry and QSAR. Ecological functions of secondary metabolites of bacteria, fungi, algae, plants, invertebrates and vertebrates. There are no other books on biochemical ecology in the international literature that analyzes so broad diversity of data on metabolites of various taxons. Published reviews indicated that the book broke a fresh ground and extended the frontiers of ecology. The new concepts suggested: ecological chemomediators and ecological chemoregulators in ecosystems]. Translated into Polish and Bulgarian languages.