This Investigation is devoted to the search for new effective anticancer drugs, which is one of the priority areas of medicine. Effect of extracts of maize anthocyanin and Gratiola officinalis drug was studied by using morphological and biochemical methods in experiments in vivo on 30 laboratory rats with transplanted liver cancer PC-1. It was found that an extract of Gratiola officinalis has selective cytotoxic and cytostatic effects on tumor cells. Zea mays extract reduces endogenous intoxication, but its effect on the tumor requires further study.
Nikita A. Navolokin, Natalya V. Polukonova, Galina N. Maslyakova, Alla B. Bucharskaya, Natalya A. Durnova Effect of extracts of Gratiola officinalis and Zea mays on the tumor and the morphology of the internal organs of rats with transplanted liver cancer // Russian Open Medical Journal, Vol. 1, Issue 2, 2012, pp. 203-0

http://scipeople.ru/publication/118623/; Toxicity of Gold Nanoparticles for Plants in Experimental Aquatic System S. A. Ostroumov, V. A. Poklonov, S. V. Kotelevtsev, and S. N. Orlov Department of Biology, Moscow State University, Moscow, Russia email: ostroumov@mail.bio.msu.ru Received April 10, 2013 Abstract: Increased production and use of nanomaterials can lead to new types of pollution of the environment, including aquatic ecosystems. Pollution of the aqueous environment with nanoparticles can be a new type of pollution of the environment. This requires a more detailed study of the biological effects during exposure of nanoparticles on aquatic organisms. The interactions of gold nanoparticles (Au) with aquatic macrophytes Ceratophyllum demersum have been studied. Aquatic microcosms with these plants were used. Gold nanoparticles (Au) were added to the aqueous medium of C. demersum macrophyte containing microcosms. The state of the plants was then analyzed. Phytotoxicity of Au nanoparticles for aquatic macrophytes was shown for the first time. A new method of phytotoxicity detection was suggested and successfully approved. Phytotoxicity at a concentration of Au (in the form of nanoparticles) of 6 . 10–6 M–1.8 . 10–5 M was shown. Original Russian Text © S.A. Ostroumov, V.A. Poklonov, S.V. Kotelevtsev, S.N. Orlov, 2014, published in Vestnik Moskovskogo Universiteta. Biologiya, 2014, No. 3, pp. 19–23. Keywords: hydrobionts, aquatic, microcosms, macrophytes, nanoparticles, Au, Ceratophyllum demersum, gold, toxicity, phytotest, phytotoxicity, nanomaterials, nanotoxicity, nanotechnology DOI: 10.3103/S0096392514030080
ISSN 0096-3925, Moscow University Biological Sciences Bulletin, 2014, Vol. 69, No. 3, pp. 108–112. © Allerton Press, Inc., 2014.

The purpose of the work is to study antitumor activity of flavonoid-containing extracts of anthocyan maize and gratiola in experiments in vivo and to estimate their effects on the internal organs, tumors and blood of rats with transplanted liver cancer PC-1. Materials and methods: Effect of extracts of anthocyan maize and gratiola has been studied in experiments in vivo in 30 laboratory rats with transplanted liver cancer PC-1 using morphological and biochemical methods. Results: It has been found that extract of gratiola received by a certain way has selective cytotoxic effect on tumor cells. Anthocyan maize extract reduces endogenous intoxication. Conclusion: These results open the prospect of further study of the extract of gratiola in various experimental models of cancer and anthocyan maize extract use may be developed as a means of endogenous intoxication reducing for application in the treatment of cancer. Investigation of anthocyan maize extract may assist in the development of drugs that reduce the endogenous intoxication at the treatment of cancer.
Navolokin N.A., Polukonova N.V., Maslyakova G.N., Bucharskaya А.В., Durnova N.A. Internal organs morphology and tumors in laboratory rats with transplanted liver cancer PC-1 by oral intoduction containing extract of Gratiola (Gratiola Officinalis L.) and Anthocyan Maize (Zea Mays L.) // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 2, 2013, pp. 213-220

The purpose of the article is to assess the severity of the morphological changes in the internal organs of laboratory animals with prolonged oral administration of gold nanoparticles of different sizes. Material and methods: The experiment was carried out on 40 albino rats which were divided into 4 groups. Animals of the experimental group received oral gold nanoparticles by the appropriate scheme. Results: At comparing the morphological changes in the internal organs of laboratory animals it was established that the introduction of gold nanoparticles of different sizes leads to the same type of disturbances of morphological structure, the gold nanoparticles of 1-3 and 50 nm cause the most expressive changes. Conclusion: The most significant morphological modifications in the internal organs were caused by gold nanoparticles of 1-3 and 50 nm. It points out to the size-dependence of the effect of gold nanoparticles.
Maslyakova G.N., Pakhomiy S. S., Bucharskaya А.В., Zlobina O.V., Navolokin N.A., Ponukalin A.N., Khlebtsov N.B., Khlebtsov B.N., Bogatyrev V.A. Morphological changes in the organs of laboratory animals during the prolonged oral administration of gold nanoparticles // Saratov Journal of Medical Scientific Research, Vol. 9, Issue 2, 2013, pp. 208-213

http://scipeople.com/publication/108106/; Solomonova E.A., Ostroumov S.A. Tolerance of an aquatic macrophyte Potamogeton crispus L. to sodium dodecyl sulphate. - Moscow University Biological Sciences Bulletin, 2007. 62(4):176-179. (in English); Abstract: The effects of the anionic surfactant sodium dodecyl suplphate on the aquatic macrophyte Potamogeton crispus L. were studied. Concentrations of 83–133 mg/l caused fragmentation of the stems of plants. The tolerance of the plants to the negative effects of the surfactant was higher in the spring (April) than in the autumn (September). DOI:10.3103/S0096392507040074. Allerton Press, Inc. Distributed worldwide by Springer. ISSN 0096-3925; Key words: Tolerance, Aquatic, Macrophyte, Potamogeton crispus, toxicity, anionic surfactant, Sodium Dodecyl Sulphate, phytotoxicity, plant science, on the journal: http://www.maik.ru/cgi-perl/journal.pl?lang=eng&name=bbscimgu&page=board; Table of contents of this issue of the journal: http://www.maik.ru/contents/bbscimgu/bbscimgu4_7v62cont.htm
Moscow University Biological Sciences Bulletin, 2007. 62(4):176-179

Objective: Morphological changes of internal organs and brain of laboratory animals have been studied at prolonged oral administration of iron nanoparticles in experiments. Materials and methods: Iron nanoparticles 70±10 nm, 28 male white rats, and morphological methods of evaluation have been used in the experiment. Results: Studies have shown that the changes depend on nanoparticle concentration and signs of circulatory disorders and degenerative changes of parenchymatous elements
Navolokin N.A., Kun S.M. Morphological changes of internal organs and brain at prolonged oral administration of iron nanoparticles // Saratov Journal of Medical Scientific Research, Vol. 7, Issue 4, 2011, pp. 760-762

Ostroumov S.A., Kotelevtsev S.V. Toxicology of nanomaterials and environment. - Ecologica. 2011, vol. 18, issue 61, pp. 3-10. Manufactured nanoparticles demonstrated a variety of biological activities, including toxicity. The goal of this review articles is to summarize some evidence of a variety of toxic effects produced by manufactured nanoparticles, including both the data from literature and the new data of the authors (e.g., [42]). The toxicity of nanomaterials was shown both to prokaryotic and eukaryotic organisms. As for eukaryotic organisms, toxicity was found in bioassays with both animal and plant test systems. In further studies, it is necessary to continue the studies of various aspects of toxicity of nanoparticles, and to extend the range of organisms and test systems that are being used for assessing the biological effects of nanomaterials. The methods that were previously developed to study phytotoxicity of chemicals [15-28, 48] will be useful to generate new data on toxicology of nanomaterials.
Ecologica. 2011, vol. 18, issue 61, pp. 3-10.

Possible employment of oxide nanostructures in medicine demands elucidation of their negative effects on various systems of a human organism. In particular, in this paper the experimental results of investigation of interaction of Ag, Cu, Fe, and Pt nanostructures water dispersions (NWD), which were produced by means the treatment of water by pulsed electric discharges (PED), with serum of human blood (HBS), are presented. The goal of the investigation was to determine connection between the properties of the nanostructures and peculiarities of their interactions with the human blood serum (HBS) as highly concentrated solutions of macromolecules. It was found that albuminous and lipoprotein structures of HBS are agglutinated on surface of the nanoparticles, forming upper molecule complexes. Thus, the higher the concentration of nanoparticles in joint dispersion is, the larger complexes are formed. At early stages of the process rather small complexes of albuminous and immunoglobulins are formed, then lipoproteins are agglutinated, and finally, large complexes of hydrodynamic radius (Rh) more than 2 μm are formed.
High Temperature Material Processes, 2009, Volume 13 / Issue 3-4, pp. 325-334

In the existing QSAR methods any molecule is represented as a single point in many-dimensional space of molecular descriptors. We proposed a new QSAR approach based on Quantitative Neighbourhoods of Atoms (QNA) descriptors, which characterize each atom of a molecule and depend on the whole molecule structure. In “Star Track” methodology any molecule is represented as a set of points in two-dimensional space of QNA descriptors. By our new method the estimate of target property of chemical compound is calculated as the average value of the function of QNA descriptors in the points of the atoms of a molecule in QNA descriptors’ space. Substantially, we have proposed to use only two instead of more than three thousand molecular descriptors applying in QSAR. On the basis of this approach we have developed computer program GUSAR and compared it with the several widely used QSAR methods including CoMFA, CoMSIA, Golpe/GRID, HQSAR and others, using ten data sets representing various chemical series and diverse types of biological activity. It was shown that in the majority of cases the accuracy and predictivity of GUSAR models appeared to be better than for the reference QSAR methods. High predictive ability and robustness of GUSAR were also shown in leave-20%-out cross-validation procedure.
SAR and QSAR in Environmental Research, 2009, 20 (7-8), 679-709.