The diagnostic significance of molecular markers was evaluated by immunohistochemical research of surgical and biopsy material of 106 patients with diseases of bladder and 57 patients with diseases of prostate. They received treatment at Scientific Research Institute of Fundamental and Clinical Uronephrology with monoclonal antibodies: Ki-67, PCNA, p63 (markers of proliferative activity), p53 (suppressor of tumor growth), Bcl-2, Bax (markers of apoptosis), EGFR (receptor of epidermal growth factors), PSA, PSMA, AMACR (prostate-specific antigens) and cytokeratin profile. The morphometric research was conducted on the basis of automated analysis system of digital images Ariol SL50 (Genetix)
Popkov V.M., Ponukalin A.N., Maslyakova G.N., Tsmokalyuk E.N., Bucharskaya A.B., Shirokov A.A., Burov A.M. Application of automated morphometric method of estimation of expression of immunohistochemical markers in diagnostics of prostate and bladder cancer // Saratov Journal of Medical Scientific Research, Vol. 6, Issue 4, 2010, pp. 845-849

In scientific article the research goal is the comparative analysis of data of clinical, laboratory and radiation diagnostic techniques of prostate cancer with histomorphological tumor gradation by Gleason scale. the level of prostate-specific antigen (PSA) and index PSA D depend on parameters on Gleason scale. Ultrasonic findings are correlated with histomorphological gradation of adenocarcinoma. Magnetic-resonance imaging is effective only with 8-10 points on Gleason scale. In the research 217 patients with suspecting prostate cancer have been included. As a result prostate cancer has been diagnosed in 157 (72,4%) patients, prostate adenoma - in 60 (27,6%) patients
P.V. Glybochko, T.G. Hmara, V.M. Popkov, M.L. Chekhonatskaya, G.N. Maslyakova, V.N. Priezsheva, A.N. Ponukalin Comparative analysis of data of clinical, laboratory and radiation diagnostic techniques of prostate cancer with histomorphological tumor gradation by Gleason scale // Saratov Journal of Medical Scientific Research, Vol. 6, Issue 2, 2010, pp. 446-453

The scientific article points out that 40 sessions of HIFU prostate ablation have been performed for estimation of clinical efficiency. Average frequency of influences presents 628±164 impulses; average volume of tissues subjected to influence during one procedure is 33,8±16,3 smi (132 %) of prostate volume; average operation time constitutes 150 minutes (from 90 to 200 minutes). During the operation no complications have been occurred. In the first days after the session of HIFU there was a peak of PSA increase and then during 1,5-3 months there was decrease to the lowest index. Minimal PSA level was reached in 10-12 weeks after treatment and it constituted from 0,04 till 1,1 ng\ml depending on the disease state. Reduction of prostate volume occurred in average from the 30th day of postoperative period and lasted for 6 months, reaching in average 50 % from initial volume. Postoperative period varied from 10 till 16 days and constituted in average 12±0,8 days. Thus application of high-intensity focused ultrasonic ablation allows treating successfully various stages of prostate cancer with minimal number of side-effects and makes possible the early estimation of treatment efficiency
P.V. Glybochko, R.N. Fomkin, A.N. Ponukalin, B.I. Blumberg, A.V. Stativko First Experience Of Application Of High-Intensity Focused Ultrasonic Ablation (Hifu) In Prostate Cancer Treatment // Saratov Journal of Medical Scientific Research, Vol. 5, Issue 4, 2009, pp. 599-603